IB Biology HL Theme A: Unity and diversity questions

Exam-style multiple choice and written questions with full markschemes. Check multiple choice answers straight away, or type, photograph or draw a written answer and get it marked.

Question 1

Paper 2Medium16 marks

A student investigated the effect of water availability on seed germination. Five dishes were used for each treatment, with 20 seeds in each dish. Each dish contained filter paper and the same type of water. The dishes were kept at the same temperature and light conditions. After 72 hours, a seed was counted as germinated if its radicle had emerged. At 20 mL, the seeds were fully submerged; at the other volumes, the filter paper was moist but the seeds were not submerged.

Water added / mLNumber of seeds germinated out of 100
00
238
582
1086
2042
(a)

Calculate the difference in germination percentage points between the 10 mL and 20 mL treatments.

[2]
(b)

Describe the relationship between water added and germination.

[3]
(c)

Explain why germination was absent without added water but high in the 5 mL and 10 mL treatments.

[3]
(d)

Explain how water acts as a transport medium from roots to leaves in a seedling, including one adaptation of xylem for this role.

[3]
(e)

The syllabus statement is that water is the medium for life. Using the results and your biological knowledge, explain how water can be essential as a medium without increasing water volume always increasing germination. Refer to the environment in which the first cells originated and to a role of water in present-day cells.

[5]

Question 2

Paper 2Medium16 marks

A student makes a temporary mount of leaf epidermal cells and examines it using a light microscope. At the objective used, a length of 100 μm on a stage micrometer spans 40 eyepiece graticule divisions. One cell has a width of 24 eyepiece graticule divisions. A printed image of the same cell has a width of 6.0 mm. Assume that the printed image has not been resized.

(a)

Calculate the actual length represented by one eyepiece graticule division.

[2]
(b)

Calculate the actual width of the cell and the magnification of the printed image. State the length on the printed image of a scale bar representing 10 μm.

[5]
(c)

A fluorescent stain is used to make cell boundaries visible. To locate protein R, a fluorescently labelled antibody against R is used. Describe how immunofluorescence reveals the location of R and distinguish it from the use of the fluorescent stain.

[3]
(d)

In a further test, 100 cells were scored according to the location of the strongest detectable fluorescence signal.

PreparationPlasma membrane (%)Cytoplasm (%)No detectable signal (%)
Fluorescently labelled antibody against R70255
Fluorescently labelled non-specific antibody6787

Assess the claim that protein R is located exclusively at the plasma membrane, using the data.

[3]
(e)

Another team wants to investigate the internal surfaces of cell membranes and the detailed structure of a protein complex while preserving it close to its native state. Outline how electron microscopy and its developments can be used to address these aims.

[3]

Question 3

Paper 2Medium4 marks

A culture of Escherichia coli containing bacteriophage lambda was monitored. At 40 min, the culture conditions were changed. Figure 1 shows the measured concentrations of intact bacterial cells and free lambda particles.

02040608001234567Time after start of monitoring / minConcentration / 106 mL-1● Intact bacterial cells□ Free lambda particles
(a)

Using Figure 1, identify the viral cycle most consistent with each interval, 0–40 min and 40–80 min. Support each choice with one feature of the graph.

[2]
(b)

Explain how lambda DNA is maintained during the first interval, and state two ways in which the bacterial host supports phage production during the second interval.

[2]

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