IB Biology HL Theme A: Unity and diversity questions

Exam-style multiple choice and written questions with full markschemes. Check multiple choice answers straight away, or type, photograph or draw a written answer and get it marked.

Question 1

Multiple choiceHard1 mark

For one star, a model places the Goldilocks zone—the orbital band where surface conditions could permit liquid water—at 1.20–2.00 × 10¹¹ m. A survey retains a site only if it is in this band, has liquid water and shows the expected solubility pattern for known hydrophilic and hydrophobic probes. Which site should be retained?

SiteOrbital distance / (10¹¹ m)Water observedHydrophilic probe in waterHydrophobic probe in water
A1.50LiquidDissolvedDissolved
B1.70IceNo liquid sampleNo liquid sample
C1.80LiquidDissolvedUndissolved
D2.20LiquidDissolvedUndissolved
Show answer and explanation

Answer: C. The modeled zone spans 1.20–2.00 × 10¹¹ m, so site C at 1.80 × 10¹¹ m is within it. Its sample is liquid, with the hydrophilic probe dissolved and the hydrophobic probe undissolved, matching water’s solvent properties. Many hydrophilic molecules dissolve in water, and most enzymes catalyse reactions in aqueous solution, so these observations support a possible medium for metabolism, but do not establish that life is present.
A: the location and liquid water fit, but dissolution of the known hydrophobic probe does not match the expected solvent pattern.
B: being in the zone does not make ice a liquid aqueous medium for enzyme catalysis.
D: the solvent observations fit, but 2.20 × 10¹¹ m is beyond the survey’s zone.
Remember: the Goldilocks zone makes surface liquid water possible; it does not guarantee water or life.

Question 2

Multiple choiceEasy1 mark

During molecular visualization of chromatin, a student selects a DNA–protein unit for closer study. Which arrangement in the model represents a nucleosome?

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Answer: C. A nucleosome has DNA wrapped around a core of eight histone proteins, with an additional histone attached to linker DNA.

A: uses seven histone proteins in the core instead of eight. B: places the additional histone on the core rather than on linker DNA. D: omits the additional histone attached to linker DNA.

Remember: distinguish the eight-protein core from the additional linker-associated histone.

Question 3

Multiple choiceMedium1 mark

A study finds the same genetic code and shared genes across bacteria, archaea and eukaryotes. The oldest known fossil evidence of cells is dated to 3.5 billion years ago, while a molecular-clock estimate places LUCA at 3.8 billion years ago. Which interpretation is supported?

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Answer: B. A universal genetic code and genes shared across the three domains support common ancestry and LUCA. A molecular clock estimates LUCA’s age, while fossils provide evidence that cells existed by the fossil date, not the exact beginning of life. Earlier lineages could have become extinct through competition with LUCA and its descendants. A: Treats the oldest known fossil as the exact origin of life; fossil evidence does not establish that life began then. C: Reverses the dating approaches: molecular clocks estimate LUCA’s age, while fossils provide evidence of ancient cells. D: Equates LUCA with the first life and treats its estimated age as excluding earlier forms. Remember: LUCA is the latest common ancestor of extant life, not necessarily the first life.

Question 4

Multiple choiceMedium1 mark

A filamentous eukaryote has a cell wall containing chitin. Microscopy of one tube shows many nuclei in a continuous length of cytoplasm, with no cross-walls. Which interpretation accounts for both observations?

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Answer: B. Chitin is characteristic of fungal cell walls. The absence of cross-walls and presence of many nuclei in continuous cytoplasm identify an aseptate fungal hypha.

A: associates a cell wall with plants but overlooks that plant walls contain cellulose, not chitin.
C: matches the multiple nuclei but overlooks that skeletal muscle cells have no cell wall.
D: identifies a fungus from the chitin wall but contradicts the observation that there are no cross-walls.
Remember: aseptate fungal hyphae contain multiple nuclei in continuous cytoplasm.

Question 5

Multiple choiceMedium1 mark

Bacteriophage lambda is undergoing its lytic cycle in bacterial cells. The table shows mean measurements at successive times after infection, all normalized per initially infected bacterium.

Time after infection / minPhage DNA copies per initially infected bacteriumAssembled phages inside bacteriaFree infectious phages
0100
102800
2028200
3028018

Which interval is most consistent with release of assembled phages from the bacteria?

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Answer: B. From 20 to 30 min, assembled phages inside bacteria fall from 20 to 0 while free infectious phages rise from 0 to 18. This indicates release; the phage DNA copy number remains unchanged during this interval.

A: selects the earlier increase in phage DNA, which indicates replication rather than release.
C: identifies the interval when assembled particles appear inside bacteria, indicating assembly before release.
D: assigns replication to an interval when the phage DNA copy number is unchanged.

Remember: distinguish replication, assembly and release by tracking what changes inside and outside the host cell.

Question 6

Multiple choiceMedium1 mark

The key is used for three local pond species. A field team identifies an observed animal and compares a barcode from pond-water environmental DNA (eDNA) with reference barcodes. The animal has no shell and six legs; the sample barcode is CTAG. Which pair gives the animal’s key identification and the species detected in the water sample, respectively?

Dichotomous key

FeatureIdentification or route
Shell presentPond snail
Shell absentGo to 2
Six legsWater beetle
More than six legsFreshwater shrimp

Reference barcodes

SpeciesBarcode
Pond snailGACT
Water beetleTGCA
Freshwater shrimpCTAG
Show answer and explanation

Answer: A. The key routes an animal with no shell to the next distinction, where six legs identifies a water beetle. The eDNA barcode CTAG matches the freshwater shrimp reference barcode.

B: identifies the observed animal correctly but assigns the sample barcode to the pond snail, whose barcode is GACT.
C: matches the sample barcode correctly but selects the shell-present route for the observed animal.
D: swaps the key identification and the species identified from the eDNA barcode.
Remember: a dichotomous key uses observable features; an eDNA barcode is compared with reference barcodes.

Question 7

Multiple choiceHard1 mark

A museum’s catalogue covers many species and must organize them for comparative studies while reflecting inferred ancestry. The table gives the branching sequence, from earliest split to latest, and current class assignments. A clade contains a common ancestor and all its descendants. Which proposal meets both aims?

InformationData
Branching sequenceS separates from P, Q and R; then R separates from P and Q; then P separates from Q
Current classesM: P and Q; N: R and S
Show answer and explanation

Answer: B. A clade contains a common ancestor and all its descendants. The branching sequence gives nested clades P+Q and P+Q+R (and the full set); R+S is not a clade because their common ancestor also has P and Q as descendants. Classification can still organize extensive diversity for comparison, while unranked clades avoid implying that fixed taxonomic ranks measure evolutionary divergence.
A: It assumes both traditional classes are clades; class N excludes P and Q even though they descend from the same ancestor as R and S.
C: It treats the limitation of fixed ranks as a reason to discard classification, although classification facilitates comparative study.
D: It assigns rank by group size; the branching pattern does not require nested clades to have class and order ranks.
Remember: clades express common ancestry, whereas taxonomic ranks are fixed categories.

Question 8

Multiple choiceHard1 mark

Researchers recorded responses to courtship displays between and within two species, then recorded the outcomes of resulting matings. Evolution is a change in heritable characteristics of a population. Which interpretation is supported?

PairingCourtship displays accepted (%)Matings producing viable offspring (%)Viable offspring that were fertile (%)
P × P849296
Q × Q819095
P × Q8880
Show answer and explanation

Answer: C. Courtship displays were accepted less often between species than within either species, indicating a behavioural barrier to mating. Although some interspecific matings produced viable offspring, none of the viable hybrids were fertile, so they could not pass alleles to later generations. The results indicate barriers to allele flow but do not show a change in heritable characteristics of a population over time.

A: Confuses viability with fertility; the hybrids were viable but could not reproduce.
B: Reverses the barrier stages: courtship affects mating before fertilization, while sterility occurs in hybrids.
D: Treats a difference between pairings as evidence of heritable change over time; the data do not establish that change.

Remember: Viable offspring can still be sterile and unable to transmit alleles to another generation.

Question 9

Multiple choiceMedium1 mark

A conservation team compares four candidate species. The tree shows their relationships and relative branch lengths; the status labels show whether each species is globally endangered. Which candidate best fits the EDGE programme’s conservation-prioritization rationale?

Species KNot globally endangeredSpecies LGlobally endangeredSpecies MGlobally endangeredSpecies NNot globally endangered012345678910Relative evolutionary history (units)
Show answer and explanation

Answer: B. EDGE prioritization considers both evolutionary distinctness and global endangerment. The tree shows that Species L has a longer branch not shared with close relatives than Species M: 5 units compared with 1 unit. Both are globally endangered, whereas Species K’s longer independent branch is labelled not globally endangered. Species L therefore fits both considerations.

A: selects the longest independent lineage but overlooks that Species K is not globally endangered.
C: uses the global-endangerment label but overlooks that Species M shares most of its recent lineage with Species N.
D: could result from misreading Species N’s status; it is not globally endangered and has only a short unshared branch.

Remember: EDGE combines evolutionary distinctness with global endangerment.

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