Question 1

Multiple choiceHard1 mark

A prokaryote was cultured with identical mineral nutrients and no organic compounds; CO₂ availability was controlled as shown. The table gives the net change in biomass after 24 h. Which conclusion is supported?

LightCO₂ suppliedNet biomass change / mg in 24 h
YesYes+12
YesNo−2
NoYes−2
NoNo−2
Show answer and explanation

Answer: B. Photosynthetic nutrition uses light energy to make organic matter from inorganic carbon. Biomass increases only when both light and CO₂ are supplied (+12 mg in 24 h); each other condition gives a decrease. With no organic compounds supplied, this supports photosynthetic nutrition.

A: treats CO₂ as sufficient without light; the dark, CO₂-supplied culture loses biomass.
C: treats light as sufficient without CO₂; the illuminated culture without CO₂ loses biomass.
D: assumes either factor alone is sufficient; both single-factor conditions show biomass loss.
Remember: photosynthetic nutrition uses light energy and an inorganic carbon source.

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