IB Biology HL Theme A: Unity and diversity questions

Exam-style multiple choice and written questions with full markschemes. Check multiple choice answers straight away, or type, photograph or draw a written answer and get it marked.

Question 1

Multiple choiceMedium1 mark

A study finds the same genetic code and shared genes across bacteria, archaea and eukaryotes. The oldest known fossil evidence of cells is dated to 3.5 billion years ago, while a molecular-clock estimate places LUCA at 3.8 billion years ago. Which interpretation is supported?

Show answer and explanation

Answer: B. A universal genetic code and genes shared across the three domains support common ancestry and LUCA. A molecular clock estimates LUCA’s age, while fossils provide evidence that cells existed by the fossil date, not the exact beginning of life. Earlier lineages could have become extinct through competition with LUCA and its descendants. A: Treats the oldest known fossil as the exact origin of life; fossil evidence does not establish that life began then. C: Reverses the dating approaches: molecular clocks estimate LUCA’s age, while fossils provide evidence of ancient cells. D: Equates LUCA with the first life and treats its estimated age as excluding earlier forms. Remember: LUCA is the latest common ancestor of extant life, not necessarily the first life.

Question 2

Multiple choiceMedium1 mark

A filamentous eukaryote has a cell wall containing chitin. Microscopy of one tube shows many nuclei in a continuous length of cytoplasm, with no cross-walls. Which interpretation accounts for both observations?

Show answer and explanation

Answer: B. Chitin is characteristic of fungal cell walls. The absence of cross-walls and presence of many nuclei in continuous cytoplasm identify an aseptate fungal hypha.

A: associates a cell wall with plants but overlooks that plant walls contain cellulose, not chitin.
C: matches the multiple nuclei but overlooks that skeletal muscle cells have no cell wall.
D: identifies a fungus from the chitin wall but contradicts the observation that there are no cross-walls.
Remember: aseptate fungal hyphae contain multiple nuclei in continuous cytoplasm.

Question 3

Multiple choiceMedium1 mark

Bacteriophage lambda is undergoing its lytic cycle in bacterial cells. The table shows mean measurements at successive times after infection, all normalized per initially infected bacterium.

Time after infection / minPhage DNA copies per initially infected bacteriumAssembled phages inside bacteriaFree infectious phages
0100
102800
2028200
3028018

Which interval is most consistent with release of assembled phages from the bacteria?

Show answer and explanation

Answer: B. From 20 to 30 min, assembled phages inside bacteria fall from 20 to 0 while free infectious phages rise from 0 to 18. This indicates release; the phage DNA copy number remains unchanged during this interval.

A: selects the earlier increase in phage DNA, which indicates replication rather than release.
C: identifies the interval when assembled particles appear inside bacteria, indicating assembly before release.
D: assigns replication to an interval when the phage DNA copy number is unchanged.

Remember: distinguish replication, assembly and release by tracking what changes inside and outside the host cell.

Question 4

Multiple choiceMedium1 mark

The key is used for three local pond species. A field team identifies an observed animal and compares a barcode from pond-water environmental DNA (eDNA) with reference barcodes. The animal has no shell and six legs; the sample barcode is CTAG. Which pair gives the animal’s key identification and the species detected in the water sample, respectively?

Dichotomous key

FeatureIdentification or route
Shell presentPond snail
Shell absentGo to 2
Six legsWater beetle
More than six legsFreshwater shrimp

Reference barcodes

SpeciesBarcode
Pond snailGACT
Water beetleTGCA
Freshwater shrimpCTAG
Show answer and explanation

Answer: A. The key routes an animal with no shell to the next distinction, where six legs identifies a water beetle. The eDNA barcode CTAG matches the freshwater shrimp reference barcode.

B: identifies the observed animal correctly but assigns the sample barcode to the pond snail, whose barcode is GACT.
C: matches the sample barcode correctly but selects the shell-present route for the observed animal.
D: swaps the key identification and the species identified from the eDNA barcode.
Remember: a dichotomous key uses observable features; an eDNA barcode is compared with reference barcodes.

Question 5

Multiple choiceMedium1 mark

A conservation team compares four candidate species. The tree shows their relationships and relative branch lengths; the status labels show whether each species is globally endangered. Which candidate best fits the EDGE programme’s conservation-prioritization rationale?

Species KNot globally endangeredSpecies LGlobally endangeredSpecies MGlobally endangeredSpecies NNot globally endangered012345678910Relative evolutionary history (units)
Show answer and explanation

Answer: B. EDGE prioritization considers both evolutionary distinctness and global endangerment. The tree shows that Species L has a longer branch not shared with close relatives than Species M: 5 units compared with 1 unit. Both are globally endangered, whereas Species K’s longer independent branch is labelled not globally endangered. Species L therefore fits both considerations.

A: selects the longest independent lineage but overlooks that Species K is not globally endangered.
C: uses the global-endangerment label but overlooks that Species M shares most of its recent lineage with Species N.
D: could result from misreading Species N’s status; it is not globally endangered and has only a short unshared branch.

Remember: EDGE combines evolutionary distinctness with global endangerment.

The best questions are still waiting

Unlock every question in this topic, then type, photograph or draw your answer and have it marked against the markscheme. Find out where you are dropping marks.